Searching ICSI Treatment Centre in Ravet, Punawale, Somatane Phata, PCMC, Pune? Conceive IVF, Fertility And Test Tube Baby Center is the Best ICSI Treatment Centre in Ravet, Punawale, Kiwale, Somatane Phata, Gahunje, PCMC and Pune. An alternative method of egg fertilization is called Intracytoplasmic Sperm Injection, or ICSI. An extremely sharp glass needle is used to inject one normal appearing sperm directly into the center (cytoplasm) of the egg under the guidance of a specially fitted microscope and the micromanipulator.